MotP Subunit is Critical for Ion Selectivity and Evolution of a K+-Coupled Flagellar Motor

MotP Subunit is Critical for Ion Selectivity and Evolution of a K+-Coupled Flagellar Motor

Blog Article

The bacterial flagellar motor is a sophisticated nanomachine embedded in the cell envelope.The flagellar Bike Parts - Cassettes motor is driven by an electrochemical gradient of cations such as H+, Na+, and K+ through ion channels in stator complexes embedded in the cell membrane.The flagellum is believed to rotate as a result of electrostatic interaction forces between the stator and the rotor.In bacteria of the genus Bacillus and related species, the single transmembrane segment of MotB-type subunit protein (MotB and MotS) is critical for the selection of the H+ and Na+ coupling ions.

Here, we constructed and characterized several hybrid stators combined with single Na+-coupled and dual Na+- and K+-coupled stator subunits, and we report that the MotP subunit is critical for the selection of BATH BOMB REVIVAL K+.This result suggested that the K+ selectivity of the MotP/MotS complexes evolved from the single Na+-coupled stator MotP/MotS complexes.This finding will promote the understanding of the evolution of flagellar motors and the molecular mechanisms of coupling ion selectivity.